This happens if you accidentally created a standard data ISO instead of an Xbox-compatible ISO. Ensure you used an Xbox-specific tool like extract-xiso or C-Xbox Tool rather than generic software like ImgBurn or UltraISO. Xbox emulators require the specific Xbox filesystem (FatX) layout inside the image.
To extract to a specific directory: extract-xiso "Game.xiso" -d "C:/Extracted Games/Game" . convert xiso to iso repack